In order to study the effect of water stress on OIL PERCENT and OIL yield in two rape seed cultivars, the experiment conducted into factorial split plots design in the basis of complet randomized block with 3 replications in two regions (Pakdasht and Karaj).There were two factors, irrigation intervals (45, 64 and 85 milimeter evaporated of pan) and water amounts in 3 levels (60, 80, and 100 PERCENT of evaporation) into factorial as main factor and cultivars in 2 levels (Talayieh and PF7045.91) as sub-factor.The results indicated that interaction effects of irrigation intervals and water amounts had significant difference at 1% level on OIL PERCENT, OIL yield and seed yield in two regions. After irrigation interval decreased and water consumption increased to 80%, maximum amount of OIL PERCENT, OIL yield and seed yield was 40.93%kg/ha, 1044kg/ha and 2538kg/ha. interaction effect of irrigation intervals and cultivars had significant difference at 1% level. when irrigation intervals or water stress intensity reduced, OIL PERCENT, OIL yield and seed yield reduced, hut these amounts in PF7045.91 was greater than talayieh the maximum OIL yield of PF7045.91 and talayieh was 1096kg/ha and 807kg/ha, OIL PERCENT was 41/39% and 39/36% and seed yield was 2640kg/ha and 2050kg/ha. interaction effect of water amounts and cultivars showed significant difference at 1% level of probability. when water amount increased to 80%, OIL yields of PF7045.91 and talayieh increased to 944/2kg/ha and 707.0kg/ha, OIL PERCENT increased to 40/38% and 38/71% and seed yield increased to 2311 and 1816 kg/ha.